LOCUS       sequence-123130-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The artificial microRNA is designed for down-regulating expression 
            of ACD6 mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Todesco M, Balasubramanian S, Hu TT, Traw MB, Horton M, Epple P, 
            Kuhns C, Sureshkumar S, Schwartz C, Lanz C, Laitinen RA, Huang Y, 
            Chory J, Lipka V, Borevitz JO, Dangl JL, Bergelson J, Nordborg M, 
            Weigel D
  TITLE     Natural allelic variation underlying a major fitness trade-off in 
            Arabidopsis thaliana.
  JOURNAL   Nature. 2010 Jun 3;465(7298):632-6. doi: 10.1038/nature09083.
  PUBMED    20520716
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-06-3- 3"; volume: "465"; issue: "7298"; pages:
            "632-6"
COMMENT     Sequence Label: MT79
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     228..248
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        230..248
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        287..305
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     287..304
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
      241 actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgcagattt aggtgacact
      301 atagaatatc actagtaagc tagcttgcat gccggtcctg ctgagcctcg acatgttgtc
      361 gcaaaattcg ccctggaccc gcccaacgat ttgtcgtcac tgtcaaggtt tgacctgcac
      421 ttcatttggg gcccacatac accaaaaaaa tgctgcataa ttctcggggc agcaagtcgg
      481 ttacccggcc gccgtgctgg accgggttga atggtgcccg taactttcgg tagagcggac
      541 ggccaatact caacttcaag gaatctcacc catgcgcgcc ggcggggaac cggagttccc
      601 ttcagtgagc gttattagtt cgccgctcgg tgtgtcgtag atactagccc ctggggcact
      661 tttgaaattt gaataagatt tatgtaatca gtcttttagg tttgaccggt tctgccgctt
      721 tttttaaaat tggatttgta ataataaaac gcaattgttt gttattgtgg cgctctatca
      781 tagatgtcgc tataaaccta ttcagcacaa tatattgttt tcattttaat attgtacata
      841 taagtagtag ggtacaatca gtaaattgaa cggagaatat tattcataaa aatacgatag
      901 taacgggtga tatattcatt agaatgaacc gaaaccggcg gtaaggatct gagctacaca
      961 tgctcaggtt tt
//
