LOCUS       sequence-123135-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The gRNA construct is designed for constitutive expression of 
            salicylate hydroxylase for degradation of salicylic acid to 
            catechol..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Todesco M, Balasubramanian S, Hu TT, Traw MB, Horton M, Epple P, 
            Kuhns C, Sureshkumar S, Schwartz C, Lanz C, Laitinen RA, Huang Y, 
            Chory J, Lipka V, Borevitz JO, Dangl JL, Bergelson J, Nordborg M, 
            Weigel D
  TITLE     Natural allelic variation underlying a major fitness trade-off in 
            Arabidopsis thaliana.
  JOURNAL   Nature. 2010 Jun 3;465(7298):632-6. doi: 10.1038/nature09083.
  PUBMED    20520716
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-06-3- 3"; volume: "465"; issue: "7298"; pages:
            "632-6"
COMMENT     Sequence Label: MT363
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     87..103
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     88..104
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggaagttcat ttcatttgga gagaacacgg gggacgagct cgcccatcac aagtttgtac
       61 aaaaaagcag gctttcgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
      121 attcctgcag cccgggggat ccactagtga ttgaattcat gaaaaacaat aaacttggct
      181 tgcgcatcgg tatcgtcggc ggcggaattt ccggcgttgc cttagcactg gaactctgcc
      241 gctactccca tatccaggta cagctgttcg aggctgcgcc ggctttcggt gaggtcggtg
      301 ccggcgtgtc ctttggcccc aacgcggtgc gcgccattgt cggcctgggc ttgggcgagg
      361 cctacctgca ggttgccgac cgtacttcgg agccctggga ggacgtgtgg ttcgaatggc
      421 ggcgcggcag cgatgccagc tatctgggag ccaccatcgc tccgggcgtg ggccagtcct
      481 cggtacaccg ggcggatttc atcgacgccc tagtaactca cctcccagaa ggtatcgccc
      541 aattcgggaa gcgcgccacc caggtcgagc agcagggtgg cgaagtgcaa gtgctgttca
      601 ccgacggcac agagtaccgc tgcgaccttc tgatcggtgc cgacggaatc aagtcagcgc
      661 tccgtagcca tgtgctggaa ggtcaggggc tggccccaca agtgccgcga ttcagcggca
      721 cctgtgccta tcgggggatg gtcgacagcc tgcatctgcg agaagcctat cgggcccatg
      781 gcatcgacga gcacttggtg gacgtgccgc agatgtacct agggctcgac ggccatatcc
      841 tcacctttcc agtgaggaat ggcggcatca tcaacgtggt ggctttcatc tccgaccgta
      901 gcgagccgaa gccgacctgg cctgcggatg ccccttgggt gcgtgaggcg agcca
//
