LOCUS       sequence-123136-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The gRNA construct is designed for constitutive expression of 
            salicylate hydroxylase for degradation of salicylic acid to 
            catechol..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Todesco M, Balasubramanian S, Hu TT, Traw MB, Horton M, Epple P, 
            Kuhns C, Sureshkumar S, Schwartz C, Lanz C, Laitinen RA, Huang Y, 
            Chory J, Lipka V, Borevitz JO, Dangl JL, Bergelson J, Nordborg M, 
            Weigel D
  TITLE     Natural allelic variation underlying a major fitness trade-off in 
            Arabidopsis thaliana.
  JOURNAL   Nature. 2010 Jun 3;465(7298):632-6. doi: 10.1038/nature09083.
  PUBMED    20520716
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-06-3- 3"; volume: "465"; issue: "7298"; pages:
            "632-6"
COMMENT     Sequence Label: MT363
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..88
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     72..88
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    96..120
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acgtgcacaa cagaattgaa agcaaatatc atgcgatcat aggcgtctcg catatctcat
       61 taaagcagga ctctagaact agtggatccc ccatcaccac tttgtacaag aaagctgggt
      121 ctagatccac cgcgggaatt cgattctcga gcccttgacg tagcacaccc ccgccaccat
      181 gctcccaacc cagccgcgca cgggcctcgg ccaggtcagt gtcgaggtcg tggttccaca
      241 gccagtcgaa gcgggtcgcc aggttttccc ccagcagctg ctcgttcgca ccgacgacgg
      301 ggtcgcgcaa ctcgtataac tcgccggtct cccaggaggt ttgctgcacg cgacaggcac
      361 gagggcggcg caggtcgtcg taggcttcaa gcagctcggc gaggttgccg gcatcggcct
      421 gcgtatcgcc caacaggcgg gcgaggaagt aggcgtcctc aagcccttgg ccagcaccgg
      481 caccttggtg cggcagcatg gcgtgagctg cgtcgccgat caggacgacc cgaccgtgca
      541 cgtagcccgg cagctccgcc aggtcatgca gtgcccagag agttggtgcc gggatgcact
      601 ccagcagggc gcgcgcggca tccccccaac ccgcgaaggc atcgagcatc tcgcgctggc
      661 tcgcctcacg cacccaaggg gcatccgcag gccaggtcgg cttcggctcg ctacggtcgg
      721 agatgaaagc caccacgttg atgatgccgc cattcctcac tggaaaggtg aggatatggc
      781 cgtcgagccc taggtacatc tgcggcacgt ccaccaagtg ctcgtcgatg ccatgggccc
      841 gataggcttc tcgcagatgc aggctgtcga ccatcccccg ataggcacag gtgccgctga
      901 atcgcggcac ttgtggggcc agcccctgac cttccagcac atggctacgg agcgctgact
      961 tgattccgtc ggcaccgatc agaaggtcgc agcggtactc tgtgccgtcg gtgaacagca
     1021 cttgcacttc gccaccctgc tgctcgacct gggtggcgcg cttcccgaat tgg
//
