LOCUS       sequence-123137-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and 
            iRFP670.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pGK:TRL-EFS:iRFP670
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..673
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(126..145)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             674..694
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             797..991
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
ORIGIN
        1 ctcttcctca tctccgggac taactcgaac gctagctgtg cgatcgttta ccatggctag
       61 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga
      121 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg
      181 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca
      241 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc
      301 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac
      361 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca
      421 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg
      481 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac
      541 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga
      601 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg
      661 tgaaagtggg tcgccaaaaa agaagagaaa ggtcgacggc ggtggtgctt tgtctcctca
      721 gcactctgct gtcactcaag gaagtatcat caagaacaag gagggcatgg atgctaagtc
      781 actaactgcc tggtcccgga cactggtgac cttcaaggat gtatttgtgg acttcaccag
      841 ggaggagtgg aagctgctgg acactgctca gcagatcgtg tacagaaatg tgatgctgga
      901 gaactataag aacctggttt ccttgggtta tcagcttact aagccagatg tgatcctccg
      961 gttggagaag ggagaagagc cctggctggt ggagagagaa attcaccaag agacccatcc
     1021 tgattcagag actgcatttg aaatcaaatc atcagttaaa ctgggc
//
