LOCUS       sequence-123145-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid for inducible expression of shRNA;
            puromycin selection. See manual for detailed protocols. No further 
            technical support is available from the depositing laboratory..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Wiederschain D, Wee S, Chen L, Loo A, Yang G, Huang A, Chen Y, 
            Caponigro G, Yao YM, Lengauer C, Sellers WR, Benson JD
  TITLE     Single-vector inducible lentiviral RNAi system for oncology target 
            validation.
  JOURNAL   Cell Cycle. 2009 Feb 1. 8(3):498-504.
  PUBMED    19177017
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Cycle.
            2009 Feb 1. 8(3):498-504."
COMMENT     Sequence Label: Tet-pLKO-puro
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    48..66
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aatcaccata aacgtgaaat ccctatcagt gatagagact tataagttcc ctatcagtga
       61 tagagacacc ggtgtgttgt aaatgagcac acaaaataca catgctaaaa tattatattc
      121 tatgaccttt ataaaatcaa ccaaaatctt ctttttaata actttagtat caataattag
      181 aatttttatg ttcctttttg caaactttta ataaaaatga gcaaaataaa aaaacgctag
      241 ttttagtaac tcgcgttgtt ttcttcacct ttaataatag ctactccacc acttgttcct
      301 aagcggtcag ctcctgcttc aatcattttt tgagcatctt caaatgttct aactccacca
      361 gctgctttaa ctaaagcatt gtctttaaca actgacttca ttagtttaac atcttcaaat
      421 gttgcacctg attttgaaaa tcctgttgat gttttaacaa attctaatcc agcttcaaca
      481 gctatttcac aagctttcat gatttcttct tttgttaata aacaattttc cataatacat
      541 ttaacaacat gtgatccagc tgcttttttt acagctttca tgtcttctaa aactaattca
      601 taatttttgt cttttaatgc accaatattt aataccatat caatttctgt tgcaccatct
      661 ttaattgctt cagaaacttc gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa
      721 cctacaacat ttgttattcc tacatttgtg ccttttaata attctttaca atagcttg
//
