LOCUS       sequence-123149-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of HA tagged ubiquitin with K33 only, other 
            lysines mutated to arginines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Lim KL, Chew KC, Tan JM, Wang C, Chung KK, Zhang Y, Tanaka Y, Smith 
            W, Engelender S, Ross CA, Dawson VL, Dawson TM
  TITLE     Parkin mediates nonclassical, proteasomal-independent ubiquitination
            of synphilin-1: implications for Lewy body formation.
  JOURNAL   J Neurosci. 2005 Feb 23. 25(8):2002-9.
  PUBMED    15728840
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2005 Feb 23. 25(8):2002-9."
COMMENT     Sequence Label: pRK5-HA-Ubiquitin-K33
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             157..384
                     /codon_start=1
                     /product="human ubiquitin variant containing Lys-33, with 
                     all other lysines mutated to arginine"
                     /label=ubiquitin-K33
                     /translation="MQIFVRTLTGRTITLEVESSDTIDNVRARIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     regulatory      421..430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    434..568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(471..490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     525..544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        637..828
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(660..680)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     907..926
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ggtgtccact cccaggtcca actgcacctc ggttctatcg attgaattcc cgaaccgaca
       61 gtcggtctct tcaccaaggc cattcgcgcc accatgggct acccctatga tgtgcctgac
      121 tacgcagatc tcaatggtgg tggtggtggg tcgaccatgc agatcttcgt cagaacgtta
      181 accggtagaa ccataactct agaagttgaa tcttccgata ccatcgacaa cgttagagct
      241 agaattcaag acaaggaagg cattccacct gatcaacaaa gattgatctt tgccggtaga
      301 cagctcgagg acggtagaac gctgtctgat tacaacattc agagagagtc gaccttacat
      361 cttgtcttaa gactaagagg tggttgagcg gccgctaagt aagtaaggat ccccagcttg
      421 gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      481 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      541 catcaatgta tcttatcatg tctggatcgg gaattaattc ggcgcagcac catggcctga
      601 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
      661 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      721 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      781 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      841 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      901 tttttttatt tatgcagagg ccgaggccgc c
//
