LOCUS       sequence-123151-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM
  TITLE     Localization of ASH1 mRNA particles in living yeast.
  JOURNAL   Mol Cell. 1998 Oct . 2(4):437-45.
  PUBMED    9809065
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Oct . 2(4):437-45."
COMMENT     Sequence Label: pSL-MS2-12X
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    81..102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(117..122,123..140,141..147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    155..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     160..182
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     179..195
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     179..195
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        291..309
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        330..348
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        400..418
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        439..457
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        509..527
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        548..566
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        618..636
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        657..675
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        727..745
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        766..784
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        836..854
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        875..893
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
       61 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      121 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      181 ggaaacagct atgacatgat tacgaattcg aatggccatg ggacgtcgac ctgaggtaat
      241 tataacccgg gccctatata tggatcctaa ggtacctaat tgcctagaaa acatgaggat
      301 cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct gcagtattcc
      361 cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc acccatgtct
      421 gcaggtcgac tctagaaaac atgaggatca cccatgtctg cagtattccc gggttcatta
      481 gatcctaagg tacctaattg cctagaaaac atgaggatca cccatgtctg caggtcgact
      541 ccagaaaaca tgaggatcac ccatgtctgc agtattcccg ggttcattag atcctaaggt
      601 acctaattgc ctagaaaaca tgaggatcac ccatgtctgc aggtcgactc tagaaaacat
      661 gaggatcacc catgtctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc
      721 tagaaaacat gaggatcacc catgtctgca ggtcgactct agaaaacatg aggatcaccc
      781 atgtctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaacatg
      841 aggatcaccc atgtctgcag gtcgactcca gaaaacatga ggatcaccca tgtctgcagt
      901 attcccgggt tcattagatc tgcgcgcgat cgatatcagc gctttaaatt tgcgcatgct
      961 agctatagtt ctagag
//
