LOCUS       sequence-123153-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nuclease competent ST1-Cas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: M-ST1-VPR
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatcgaacc cttgccacca
      121 tgagcgacct ggtgctgggc ctggacatcg gcatcggcag cgtgggcgtg ggcatcctga
      181 acaaggtgac cggcgagatc atccacaaga acagtcgcat cttccctgct gctcaggctg
      241 agaacaacct ggtgcgccgc accaaccgcc agggtcgccg gcttgctcgc cgcaagaagc
      301 accggcgcgt gcgcctgaac cgcctgttcg aggagagcgg cctgatcacc gacttcacca
      361 agatcagcat caacctgaac ccctaccagc tgcgcgtgaa gggcctgacc gacgagctga
      421 gcaacgagga gctgttcatc gccctgaaga acatggtgaa gcaccgcggc atcagctacc
      481 tggacgacgc cagcgacgac ggcaacagca gcgtgggcga ctacgcccag atcgtgaagg
      541 agaacagcaa gcagctggag accaagaccc ccggccagat ccagctggag cgctaccaga
      601 cctacggcca gctgcgcggc gacttcaccg tggagaagga cggcaagaag caccgcctga
      661 tcaacgtgtt ccccaccagc gcctaccgca gcgaggccct gcgcatcctg cagacccagc
      721 aggagttcaa cccccagatc accgacgagt tcatcaaccg ctacctggag atcctgaccg
      781 gcaagcgcaa gtactaccac ggccccggca acgagaagag ccgcaccgac tacggccgct
      841 accgcaccag cggcgagacc ctggacaaca tcttcggcat
//
