LOCUS       sequence-123157-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Cre-GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Kennedy PJ, Feng J, Robison AJ, Maze I, Badimon A, Mouzon E, 
            Chaudhury D, Damez-Werno DM, Haggarty SJ, Han MH, Bassel-Duby R, 
            Olson EN, Nestler EJ
  TITLE     Class I HDAC inhibition blocks cocaine-induced plasticity by 
            targeted changes in histone methylation.
  JOURNAL   Nat Neurosci. 2013 Apr;16(4):434-40. doi: 10.1038/nn.3354. Epub 2013
            Mar 10.
  PUBMED    23475113
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3354"; 
            journalName: "Nat Neurosci"; date: "2013-04"; volume: "16"; issue: 
            "4"; pages: "434-40"
COMMENT     Sequence Label: AAV-Cre-GFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(53..55,56..58,59..769)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(53..55,56..58,59..769)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(98..119)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(359..378)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     706..727
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttgggattcg aacatcgatt gaattccccg gggatctgca ccggtcgcca ccatggtgag
       61 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      121 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      181 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      241 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      301 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      361 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      421 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      481 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      541 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      601 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      661 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      721 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ccggtacagc
      781 tctcgacgga gaaagctcag gctctggctc agagtctgac tccatggcca atttactgac
      841 cgtacaccaa
//
