LOCUS       sequence-123159-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cyclic nucleotide reporter for bacteria or plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Wheeler JI, Freihat L, Irving HR
  TITLE     A cyclic nucleotide sensitive promoter reporter system suitable for 
            bacteria and plant cells.
  JOURNAL   BMC Biotechnol. 2013 Nov 9;13:97. doi: 10.1186/1472-6750-13-97.
  PUBMED    24206622
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-11-9"; volume: "13:97. doi"; pages: " 
            10.1186/1472-6750-13-97"
COMMENT     Sequence Label: pOPTXLUC
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..67
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             560..577
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg caaccacttt gtacaagaaa
       61 gctgggtcca tagctctttt ctttttttct tgtattatgt caaacaaagg aagatataag
      121 tttggcttta ggagttggca caaagttaaa ttaactaggt ggggagcgag gaggtactta
      181 taatgtctcg ttcattttta aaagaatcat gaaagagaaa ctagaaaaat attatccaat
      241 attaatcaac tatttctaca ttaatttttg ttagaaacta gacttcaata tcaatatcca
      301 ataaatataa atctttctaa taaaaagtaa aaaaaattct taaatcaact tttgtgtaaa
      361 aagagaaaca atgaattctt atagagaagt tcatttttaa aattatggtt ttagttatta
      421 gaaaaatatc aaaatactat tgaataaatt gtgtaactaa tttaaaactt aaatatatca
      481 aataattaag tttactaaac ttcttagtgt attagagtag tgggaagaaa tggacccact
      541 gtccaatatg tttaaaatgc atcatcatca tcatcattac aagaaaattt caactttggc
      601 ctgtggctct ggggcataaa ctattgttta ctacaatgtt ttcttgacat ttacggctga
      661 atatatatag ttgcgatttg attatgatga caatctttga cgatcttccc ttcccatcaa
      721 tgtgagttca accctttccg tcagtactag tagaaactta acattaaaag aactaatcaa
      781 gtttacaata tcatcataat tt
//
