LOCUS       sequence-123160-        9855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, 
            Yamanaka S
  TITLE     Induction of pluripotent stem cells from adult human fibroblasts by 
            defined factors.
  JOURNAL   Cell. 2007 Nov 30. 131(5):861-72.
  PUBMED    18035408
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):861-72."
COMMENT     Sequence Label: phOCT4-Luc
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5126..5135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5132..6784
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5198..5216)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    6825..6946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6836..6855)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6890..6909
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7194..7211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7365..7953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7445..7464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8124..8915,8916..8984))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8747..8766
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8985..9089)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9116..9571
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9203..9222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9413..9434
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    9702..9750
                     /note="synthetic polyadenylation signal"
     misc_feature    9764..9855
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     9804..9823
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 atcgataggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcgccc
       61 ttcactcgag gtggaggagc tgagggcact gtgggaggga tggagtgcag gcccctcagc
      121 cagccttcaa aactccattt caatacccat gtcctaaatg aacacagctc tgttgctgac
      181 cgtcctagcc actctgcttt ccttccctct cccgtgcttg gtcagccctt ttcccacttg
      241 gtgttcaagt tctgacccag ggctgagcac ccaagggctc tcaaggtggg gcatcaccta
      301 cagtcaccat ggaacacagt ggaatggtgc ctaaagccct ggtgtggagg atgtacggca
      361 gcttgatagg accagaggca gccagtttgt ctgggaggtc tttgccacca cttttccctg
      421 tcccgctagc ccccttggtg catgggactc tctgcaaagc ccctggagag gagccatccc
      481 aacatgctag gcccttgact tctatcctcc agtgtccagc taacttagct gagggatggg
      541 ctgccccagc ccctgagtcc ctggaaccct aagccacttg cccccttgca tggcgctgct
      601 cagatgccca gagccagcag tctgctgtga tccctttgac gtgagcatag gctctcctgt
      661 ctggtgatgc accaggctga tgctgagtct ctgtgcaacc tcagatgagc agctggggtt
      721 tggaggcatg tagcaatgca gttgccgagt gcagtggctc gatctcagct cactgcaccc
      781 tctgctgcct gagttcaagc gatttttgtt tctcagcctc ctgagtagct gggattacag
      841 gcatgcacca ccacatgcaa aaaaaacaaa acaaacaaac aaaaaaacca ccttttggta
      901 tttttagtag agacagggtt tcaccatgtt ggccaggctg gtctcgaact gctgatctga
      961 agtgatccgc ccgcctcagc ctcctaaagt gctgggatta taggcatgag ccaccgctcc
     1021 cggcctcctc ctatatctct tgatcaaact tcaggggaaa acctgccaga ttccccagga
     1081 aggatgcttg gaggtaggtg gcagggtaca gggaggccta tgccaacttc tcattgaaca
     1141 ccactcccag ctttcctacc cccatttctc ttcacaggtg tgcagagaag tctacattcc
     1201 catgtcaagt aaggggggtg ggtagatggg agggcatatg agagcggggc agcaatttta
     1261 tgtctgagtt ggaaggaagt ttagagttca ttgagtccaa atcctcttta ctaggtgagg
     1321 aaactgaggc acaggagggg gaggtgccat tagccaggga gtcagagcca ggacttagaa
     1381 cctggtccat gcctcctagt ttgttcctga ccaatcctcc cagctcaccc actccttttg
     1441 ctctcacctc tgactcccta caggctacag ctagagagtt gactcctcta tttgaggtaa
     1501 gagtgtctgg atttgggata aggtgacctc agtttgcagg gctcatgggg gagatcagag
     1561 ggtcctttgt tgagaagagg ggacttggtc cacagtgagc aaaggaattt gtctaaattg
     1621 ctattaaggg agatcatctc ccaaggtggg ccaagcctga accctcagcc ctccgtttct
     1681 tccctttctt ttgccttcag attgacaggc ctcggaagtc aaaataagtg gtttcctaga
     1741 ccgggtcgag agcaagtctc tattggtccc aactgagttt tttcagctgg tttttcaacc
     1801 aaacagcacc tcatctccca gtgaggggaa gggaaggctg ggctgagagc agcaaggctg
     1861 ctcatctcac ctctccccac ccagccatgc cagccgcctc acctggtggg gagaggtggg
     1921 cctcacctgg gtcccctggc agtgctctgt gaagggtctt gacattgcac tgtaataata
     1981 aaggtgtgtg tgaagtatct ttttatggtg actttctaaa acccagggaa tcatgggacc
     2041 agttctgatg actcagcctg gcttccagtc tcctccaggc ccaacggtgg cccccagcac
     2101 tggttggggc ctgggagagc tggccttggc tgaagtgaag ccacctaccc ttcaggcata
     2161 acaggacagt gagaaggaag gaaagcctgc ctcaacctcc catcagccct gagcacccca
     2221 gaagggggcc ggctaggagt ctaggcatgc aggaggctga cccctgactg ggctcatatc
     2281 cagccacaag gcagccaggg atccaggcac ccaccccttg tctgcgtccc tctcgggaat
     2341 gggcctcttg cccaggccag aaatacacca cctacagtac aaattataat ctaaaaacaa
     2401 gagggtggtg ttgagtgggg aaattgggga aggtgtttta ggagccacta ggaaaatggg
     2461 cagcagggac tctctggact ggcttgggaa gagcgctttt ggggaacctg gaggatggca
     2521 agctgagaaa cactggtgtg gagattccag ccaaatccca ggcctgcccc tccccctcct
     2581 ctgagaggcc gtcttcttgg cagacagcag agagatgcat gacaaaggtg ccgtgatggt
     2641 tctgtcctgg ggattgagat ggctggggag gggcctcctc ctgttccgaa gcatgttcct
     2701 cccaccccca ccaggcccca taatctacct gccttttggg cagttaaagg ccgagaagtg
     2761 aacacagctg caaccccact gccttgtaga ccttccggca gacctgtggc aggtattgaa
     2821 atgcacgcat acaattaggc tcaaaaagtc tacacagaca ggagatgggc acacgaacag
     2881 aggcaacata agagtggggg aaaagtctca aaagactcac ggatgccacc aagatgaaga
     2941 cagctggcca cgggacaccc atccccttag aaggcagata gagccactga ccccagcaga
     3001 caagcccagg cagggctgag cctggagcct gcaatgagaa gccttactta agtcgacaga
     3061 ggtcagcgtg cccagtccag acctggcctt ctggccttcg aagctgtggg gagccctggc
     3121 ccagagcccc ctctggagcc cccagactta ccccaggccc tccactgaga tcaagttttg
     3181 ggagcagaca gacaaacatc atccctcaca gacaggcatt ccgttggcta ttctcttgca
     3241 aacagaatca agcactagac cagcagcatg agcctcagga tactcaggcc aggcccagaa
     3301 aaacagaccc tgaaggggag cttagggcag ccttcctgca cgcctccaca aatcactctc
     3361 cacctcctct gcgtctttct gccagccagc cccactaaac aaagcacatc cctcaatctg
     3421 ccaggctcgg ggagggacgc acgatgaagc tggacgcctg agtcccccag aggaaggagg
     3481 aactagatac ctaggtccct gtggggggcc cttggtgccc gtctgaggct cagtctttga
     3541 ggggattgca gaggggggtt gctggagctc cttttagcgt ctctgaaggg gattctgtgt
     3601 gaggggattg ggactggggg gttggggagc aggaagcagt ccccagggga gccatccagg
     3661 cccattcaag ggttgagcac ttgtttaggg ttagagctgc cccctctggg gaccgggatt
     3721 gtccagccaa ggccattgtc ctgccccctt cccccagtcc ctcccaggct tctttgaacc
     3781 tgaagtcaga tattttttct ccacaccccc caccccctgg ttttccccac ccagggccta
     3841 gggctggagg cctgggccag ggaggtgggg gagggagaac ggggcctacc gtggtattag
     3901 atgtctgagt tttggttgag aggggagcaa ggaacctgat gtgcaggttc catagtggag
     3961 ggggcccaaa gcgggtgtct tatcactctg tttcagcaaa ggttgggaaa ctgaggccca
     4021 gtcagtccaa agtctggtcc cttgaagggg aagtagggac caacccctta gtctgttaga
     4081 tgaggagagt ctggagtctg attctggaag acggaggggt ggggggatgg gggggtgggg
     4141 ggatatagca cggaggcctt gtctggcagt ctactcttga agatggggtg aaatttggca
     4201 ggctgggcag atggtgccag gcacccaggc tgcggggtgg ctggatttgg ccagtatcgg
     4261 gatgggaatg cctaggattc tggatggatc gggggaaggc ataagggagc agctggccat
     4321 tgtgcttatg gctgttgatg cattgaggga tagcgccaca cacacattca ataaatttga
     4381 ggagctgaga gggtgactgg cccctgaagg cacagtgcca gaggtctgtg gagagggggt
     4441 caagcacctg ggttcctgaa gaacatggag gtgtgggagt gattccagac agctgggatg
     4501 tgcagagcct gagagagtgc cagggagcgg gttgggagtt gaaagttggg tgtggtggct
     4561 cacgccttta atcatgacac tgggcggcag aggcgggagg atttcttgag gacaggaatt
     4621 caagaccagc ctgggtaaca tagcaaggcc ccatctctac taaaaataaa aaaactaaca
     4681 gggcacagtg gtccaagcct gtagtcccag ccacttagga ggctggagca gaaggattgc
     4741 tttggcccag tagatcgagg ctacattgag ccatcattgt actccactgc actccagtct
     4801 gggcaacaaa gtgagaccct gtcttaaaaa ataaaaataa aaaaagtttc tgtgggggac
     4861 ctgcactgag gtcctggagg ggcgccagtt gtgtctcccg gttttcccct tccacagaca
     4921 ccattgccac caccattagg caaacatcct tcgcctcagt ttctcccccc acctccctct
     4981 cctccaccca tccagggggc ggtgccagag gtcaaggcta gtgggtggga ctggggaggg
     5041 agagaggggt tgagtagtcc cttcgcaagc cctcatttca gatctgcgat ctaagtaagc
     5101 ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca taaagaaagg
     5161 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     5221 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     5281 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     5341 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     5401 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     5461 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
     5521 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
     5581 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     5641 tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa
     5701 gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct
     5761 gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca
     5821 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     5881 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     5941 agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac
     6001 cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     6061 cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag
     6121 gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct
     6181 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     6241 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact
     6301 gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     6361 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     6421 acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc
     6481 tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc
     6541 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     6601 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     6661 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     6721 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt
     6781 gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     6841 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     6901 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     6961 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     7021 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
     7081 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     7141 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     7201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7801 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8401 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8461 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8521 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8581 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8641 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8701 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8761 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8821 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8881 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8941 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9001 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9061 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     9121 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9181 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9241 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9301 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9361 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9421 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9481 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9541 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
     9601 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa
     9661 gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt
     9721 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     9781 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     9841 ccagaacatt tctct
//
