LOCUS       sequence-123161-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cyclic nucleotide reporter for bacteria or plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Wheeler JI, Freihat L, Irving HR
  TITLE     A cyclic nucleotide sensitive promoter reporter system suitable for 
            bacteria and plant cells.
  JOURNAL   BMC Biotechnol. 2013 Nov 9;13:97. doi: 10.1186/1472-6750-13-97.
  PUBMED    24206622
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-11-9"; volume: "13:97. doi"; pages: " 
            10.1186/1472-6750-13-97"
COMMENT     Sequence Label: pOPTXcGMPRELUC
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..67
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             630..647
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg caaccacttt gtacaagaaa
       61 gctgggtcca tagctctttt ctttttttct tgtattatgt caaacaaagg aagatataag
      121 tttggcttta ggagttggca caaagttaaa ttaactaggt ggggagcgag gaggtactta
      181 taatgtctcg ttcattttta aaagaatcat gaaagagaaa ctagaaaaat attatccaat
      241 attaatcaac tatttctaca ttaactgttg aaatctattt ggctctcaac tgttgaaatc
      301 tatttggctc tcaactgttg aaatctattt ggaatttttg ttagaaacta gacttcaata
      361 tcaatatcca ataaatataa atctttctaa taaaaagtaa aaaaaattct taaatcaact
      421 tttgtgtaaa aagagaaaca atgaattctt atagagaagt tcatttttaa aattatggtt
      481 ttagttatta gaaaaatatc aaaatactat tgaataaatt gtgtaactaa tttaaaactt
      541 aaatatatca aataattaag tttactaaac ttcttagtgt attagagtag tgggaagaaa
      601 tggacccact gtccaatatg tttaaaatgc atcatcatca tcatcattac aagaaaattt
      661 caactttggc ctgtggctct ggggcataaa ctattgttta ctacaatgtt ttcttgacat
      721 ttacggctga atatatatag ttgcgatttg attatgatga caatctttga cgatcttccc
      781 ttcccatcaa tgtgagttca accctttccg tcagtactag tagaaactta acattaaaag
      841 aactaatcaa gttt
//
