LOCUS       sequence-123162-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Terminator quantification vector utilizing Golden Gate Assembly and 
            amilCP. Positive colonies are green and false positives are blue. 
            Terminators are placed between GFP and RFP - induced by arabinose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     Reyna Lab - Terminator Verification Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PGR-Blue
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..28
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             35..748
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(65..93)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     688..708
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggctagcta ctagagaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac
       61 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      121 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      181 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
      241 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      301 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      361 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      421 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      481 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
      541 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
      601 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
      661 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
      721 tacacatggc atggatgaac tatacaaata ataagaattc cgactgagac cttattaggc
      781 gaccacaggt ttgcgtgcaa tggaaatttc acactgctca accgaagtgt aatccttgtt
      841 gtgattggtt acatccagtt tgcgg
//
