LOCUS       sequence-123163-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Terminator quantification vector utilizing Golden Gate Assembly and 
            amilCP. Positive colonies are green and false positives are blue. 
            Terminators are placed between GFP and RFP - induced by arabinose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     Reyna Lab - Terminator Verification Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PGR-Blue
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(465..843)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     790..809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
       61 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      841 gttcttc
//
