LOCUS       sequence-123164-         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint target selector ACTG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-ACTG1
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     156..178
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     194..210
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        237..485
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     237..257
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     408..427
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        506..581
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
       61 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
      121 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
      181 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggcc ggatccgagg
      241 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
      301 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
      361 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      421 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      481 acaccgagca ggagtacgac gagtcgtttt agagctagaa atagcaagtt aaaataaggc
      541 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttc
//
