LOCUS       sequence-123165-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint target selector ACTG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-ACTG1
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             211..264
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
       61 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      121 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      181 tacaaggtcg ctaccatcga gggatccgga gagggcagag gaagtctgct aacatgcggt
      241 gacgtcgagg agaatcctgg cccaatggac aagaagtaca gcatcggcct ggacatcggc
      301 accaacagcg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaagttc
      361 aaggtgctgg gcaacaccga ccgccacagc atcaagaaga acctgatcgg cgccctgctg
      421 ttcgacagcg gcgagaccgc cgaggccacc cgcctgaagc gcaccgcccg ccgccgctac
      481 acccgccgca agaaccgcat ctgctacctg caggagatct tcagcaacga gatggccaag
      541 gtggacgaca gcttcttcca ccgcctggag gagagcttcc tggtggagga ggacaagaag
      601 cacgagcgcc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
      661 cccaccatct accacctgcg caagaagctg gtggacagca ccgacaaggc cgacctgcgc
      721 ctgatctacc tggccctggc ccacatgatc aagttccgcg gccacttcct gatcgag
//
