LOCUS       sequence-123174-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for gene expression quantification using 
            destabilized TurboGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-2A-TurboGFP-PEST
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(187..291)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     486..508
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     500..517
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     501..517
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             573..626
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
       61 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      121 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
      181 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
      241 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
      301 ccgaaaagtg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
      361 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      421 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      481 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagctcg gtaccgctag
      541 cgggccagta cccaaaaagc ggggggtccg gagagggcag aggaagtctg ctaacatgcg
      601 gtgacgtcga ggagaatcct ggcccaggat ccatggagag cgacgagagc ggcctgcccg
      661 ccatggagat cgagtgccgc atcaccggca ccctgaacgg
//
