LOCUS       sequence-123176-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pGK:HygroR-CMV:mKate2-B_UTR
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(159..857)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     promoter        complement(936..1035)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(965..985)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg
       61 ggcgcgccgc ggtctagaag agtaattcaa ccccaaacaa caacgtttcc atgcaaggac
      121 tattactaag cagtacatct gggctggtgg tatactcatc atctgtgccc cagtttgcta
      181 gggaggtcgc agtatctggc cacagccacc tcgtgctgct cgacgtaggt ctctttgtcg
      241 gcctccttga ttctttccag tcttctgtcc acatagtaga cgccgggcat cttgaggttc
      301 ttagcgggtt tcttggatct gtatgtggtc ttcaagttgc agatcaggtg gcccccgccc
      361 acgagcttca gggccatgtc ggctctgcct tccaggccgc cgtcagcggg gtacagggtc
      421 tcggtggagg cctcccagcc gagtgttttc ttctgcatca cagggccgtt ggatgggaag
      481 ttcacccctc tgatcttgac gttgtagatg aggcagccgt cctggaggct ggtgtcctgg
      541 gtagcggtca gcacgccccc gtcttcgtat gtggtgactc tctcccatgt gaagccctcg
      601 gggaaggact gcttaaagaa gtcggggatg ccctgggtgt ggttgatgaa ggttttgctg
      661 ccgtacatga agctggtagc caggatgtcg aaggcgaagg ggagagggcc gccctcgacc
      721 gccttgattc tcatggtctg ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac
      781 ttgaagtggt ggttgttcac ggtgccctcc atgtacagct tcatgtgcat gttctcctta
      841 atcagctcgc tcaccatggt ccgcttaagc tgtagcgcta atgtcgggtc aatttcgata
      901 agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac
      961 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
     1021 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gaga
//
