LOCUS       sequence-123177-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for myc fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-myc
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..107)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(90..106)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(99..121)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        316..420
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(639..658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttctgctcgg atccccctcc actgccacca gaccccccgc tttttgggta ctggcccgct
       61 agctatcatt aggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg
      121 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      181 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      241 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcact
      301 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      361 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      421 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      481 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      541 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      601 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      661 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
//
