LOCUS       sequence-123181-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: R26TV LSL-TRL
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..91)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccgggccttt ctttatgttt ttggcgtctt ccatgggccc ggggttttct tccacgtctc
       61 ctgcttgctt taacagagag aagttcgtgg ctccggatcc taacttcccg gggagcatgt
      121 caaggtcaaa atcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg
      181 cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt
      241 ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtc
      301 tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat
      361 gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca
      421 gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt
      481 cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga
      541 ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt
      601 ccagggggca gaagtgggta tgatgcc
//
