LOCUS       sequence-123182-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for SunTag fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-1xSunTag
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..118
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(149..166)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    155..379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(510..529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgatagcta gcgggccagt acccaaaaag cggggggtct ggtggcagtg gagggggatc
       61 cgaggagctg ctgagcaaga actaccacct ggagaacgag gtggccagac tgaagaaggg
      121 cagcggctga gcggccgccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      181 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      241 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      301 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      361 gatgcggtgg gctctatgga cgcgtcagca aaaggccagg aaccgtaaaa aggccgcgtt
      421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      781 agccactggt aacaggatta
//
