LOCUS       sequence-123189-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for AviTag fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-AviTag
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(116..133)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(116..132)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(125..147)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        342..446
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgatcttctg ggcctcgaag atgtcgttca ggccggatcc ccctccactg ccaccagacc
       61 ccccgctttt tgggtactgg cccgctagct atcattaggt accgagctcg aattcactgg
      121 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      181 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      241 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
      301 atctgtgcgg tatttcacac cgcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      361 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      421 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      481 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      541 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      601 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
//
