LOCUS       sequence-123191-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for NanoLuc fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-NanoLuc
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    102..326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     315..332
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(351..863)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgttca ggggggcgga gcctatggaa aaacgccagc
       61 aacgcggcct ttttacggtt cctggccttt tgctgacgcg tccatagagc ccaccgcatc
      121 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
      181 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
      241 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
      301 agatggctgg caactagaag gcacagtcga ggctgatcag cggcggccgc tcacgccaga
      361 atgcgttcgc acagccgcca gccggtcact ccgttgatgg ttactcggaa cagcagggag
      421 ccgtcggggt tgatcaggcg ctcgtcgata attttgttgc cgttccacag ggtccctgtt
      481 acagtgatct ttttgccgtc gaacacggcg atgccttcat acggccgtcc gaaatagtcg
      541 atcatgttcg gcgtaacccc gtcgattacc agtgtgccat agtgcaggat caccttaaag
      601 tgatgatcat ccacagggta caccacctta aaaatttttt cgatctggcc catttggtcg
      661 ccgctcagac cttcatacgg gatgatgaca tggatgtcga tcttcagccc attttcaccg
      721 ctcaggacaa tcctttggat cggagttacg gacaccccga gattctgaaa caaactggac
      781 acacctccct gttcaaggac ttggtccagg ttgtagccgg ctgtctgtcg ccagtcccca
      841 acgaaatctt cgagtgtgaa gacggatccc cctccactgc caccagaccc cccgcttttt
//
