LOCUS       sequence-123193-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for tetracysteine fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TC
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(78..95)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(78..94)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..109)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        304..408
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(627..646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccccctccac tgccaccaga ccccccgctt tttgggtact ggcccgctag ctatcattag
       61 gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      121 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      181 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
      241 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcacttt tcggggaaat
      301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      421 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      481 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      541 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      601 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      661 gggcaagagc aactcggtcg ccgcatacac tattctcaga
//
