LOCUS       sequence-123199-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for TagRFP fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagRFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    103..327
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     316..333
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctgacgc gtccatagag cccaccgcat
      121 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
      181 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
      241 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
      301 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcggcggccg ctcaattaag
      361 tttgtgcccc agtttgctag ggaggtcgca gtatctggcc acagccacct cgtgctgctc
      421 gacgtaggtc tctttgtcgg cctccttgat tctttccagt ctgtggtcca catagtagac
      481 gccgggcatc ttgaggttct tagcgggttt cttggatctg tatgtggtct tgaagttgca
      541 gatcaggtgg cccccgccca cgagcttcag ggccatgtcg cttctgcctt ccaggccgcc
      601 gtcagcgggg tacagcatct cggtgttggc ctcccagccg agtgttttct tctgcatcac
      661 agggccgttg gatgggaagt tcacccctct gatcttgacg ttgtagatga ggcagccgtc
      721 ctggaggctg gtgtcctggg tagcggtcag cacgcccccg tcttcgtatg tggtgactct
      781 ctcccatgtg aagccctcag ggaaggactg cttaaagaag tcggggatgc cctgggtgtg
      841 gttgatgaag
//
