LOCUS       sequence-123200-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus expressing hypoxia-responsive GFP reporter and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Jacks T
  TITLE     Jacks Lab GMAP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HRE:GFP-pGK:Cre
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     162..183
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        267..766
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(302..325)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     699..718
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             797..817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
       61 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      121 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      181 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaaa accgctgttc
      241 ctaggaatcc cgaggcctat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
      301 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
      361 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
      421 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
      481 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
      541 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
      601 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
      661 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
      721 cacgtctgcc gcgctgttct cctcttcctc atctccggga gacccgacat tagcgctaca
      781 gcttaagcgg accatgccca agaagaagag gaaggtgtcc aatttactga ccgtacacca
      841 aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga
      901 catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg
      961 ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg ca
//
