LOCUS       sequence-123203-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing rtTA3-2A-Cre for generation of 
            GMAP-compatible gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO rtTA3-2A-Cre
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             815..868
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             875..895
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcttggtac cgagctcgga tcctaactcg aacgctagct gtgcgatcgt ttaccatgag
       61 tagactggac aagtccaagg tcattaacgg agccctggaa ctcctgaacg gggtgggcat
      121 cgagggactg acaacaagaa agctcgccca gaagcttgga gtggagcagc ctacactcta
      181 ctggcacgtc aaaaacaaga gagcccttct ggatgcactc cctatcgaaa tgctggacag
      241 acaccacacc catttttgtc ctcttgaagg ggagtcatgg caggacttcc ttcgcaacaa
      301 cgccaaatca tatcgctgtg cacttctgag tcatagagat ggcgccaaag tgcatctggg
      361 cacgcgcccc accgagaaac agtatgaaac actggagaac cagctggcct ttctctgtca
      421 acaaggattc agtcttgaga atgctctgta tgccctgtcc gccgtggggc atttcacact
      481 gggatgcgtc ctggaagagc aagaacatca ggttgccaaa gaggaaaggg agacccccac
      541 tacagattcc atgcccccac ttttgcggca ggcaatcgaa ctgttcgata gacagggtgc
      601 tgagcccgcc ttcctgttcg gattggaact tataatatgt ggtctggaaa aacagctcaa
      661 gtgtgaaagt ggtggtccaa ccgatgcttt ggacgatttt gacctggaca tgctgccggc
      721 cgacgccctc gatgatttcg accttgacat gctgcctgca gatgcattgg atgactttga
      781 cctggacatg ctgcctggtg gctccggagc cagagaggga cgaggttctt tgctgacttg
      841 cggcgatgtg gaagaaaacc cggggccagg catgcccaag aagaagcgca aagtgtctaa
      901 cctcctgaca gtgcaccaaa acctcccggc actgccagtg gatgctactt ctgacgaagt
      961 gcgcaaaaat cttatggaca tgttccgcga ccggcaagct tttagcgaac atacttggaa
     1021 gatgctgttg agtgtctgcc gctcttgggc cgcttggtgc aaactcaaca accggaagtg
     1081 gtttcccgct gagcctgagg atgttcggga ctatctcctg taccttca
//
