LOCUS       sequence-123205-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for split-TEV-C fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-cTEV
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..415)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     362..381
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(600..704)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
       61 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
      121 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
      181 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
      241 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
      301 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
      361 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
      421 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
      481 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
      541 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
      601 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
      661 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
      721 gtgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
      781 ccattcaggc tgcgcaactg ttgggaaagg gcgatcggtg cggg
//
