LOCUS       sequence-123206-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for split-TEV-C fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-cTEV
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    103..327
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     316..333
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(808..824)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(817..839)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctgacgc gtccatagag cccaccgcat
      121 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
      181 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
      241 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
      301 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcggcggccg ctcactggct
      361 gtacaccagc tcgttcatca gctgggtggc ctccttcacg ggctggaagg gctcctcggg
      421 cttgctcatg aacaccttgt ggccgcccca cagcacgctg tcggcgttca gtctccagcc
      481 gctcacccac tgctgggcct cctggttggt cagcagctcc atgaagttct tgggcacgct
      541 ggtgaagtag ttgttggtgt tggtgaagtt gctggcgctg tggatgccca cgatgaagcc
      601 gtctctggtg ctcaccaggg ggctgccgca ctggccgtcc ttggtctgaa tccagtgctt
      661 ccagaagatg ccgtcgctgc tggggaaggt gcagctggtg tcgctcacca tgctgctcat
      721 gctcttggat ccccctccac tgccaccaga ccccccgctt tttgggtact ggcccgctag
      781 ctatcattag gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga
      841 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
//
