LOCUS       sequence-123209-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for TagGFP2 fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagGFP2
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(184..288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     483..505
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     497..514
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     498..514
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
       61 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      301 aaaagtgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
      361 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      421 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      481 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta ccgctagcgg
      541 gccagtaccc aaaaagcggg gggtctggtg gcagtggagg gggatccatg agcgggggcg
      601 aggagctgtt cgccggcatc gtgcccgtgc tgatcgagct ggacggcgac gtgcacggcc
      661 acaagttcag cgtgcgcggc gagggcgagg gcgacgccga ctacggcaag ctggagatca
      721 agttcatctg caccaccggc aagctgcccg
//
