LOCUS       sequence-123210-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for TagGFP2 fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagGFP2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    99..323
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     312..329
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tgacgcgtcc atagagccca ccgcatcccc
      121 agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
      181 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
      241 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
      301 tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg cggccgctta cctgtacagc
      361 tcgtccatgc cgtgggtgtg gcagcaggcg ctgaaggact ccaggagcac catgtggtcg
      421 cgggcctcgt tgcggtcctt gctgatcttg gtctgagtgc tcaggtagtg gttgatgggg
      481 atcagcacgg ggccgtcgcc caggggcacg ttggtctggt agtggtcggc cagctgcacg
      541 ccgccgccct cgatgttgtg gcgggtcttg aagttagcct ccaggccgtt gttggccttg
      601 tcggggcgga tgtacacgtt gtggctgttg aagctgtact ccagcttgtg gcccaggatg
      661 ttgccgtcct ccttgaagtc cttgcccttc agctcgatgc ggttcaccag ggtgtcgccc
      721 tcgaacttca cctcgccgcg ggtcttgtac ttgccgtcgt cctggaactg gatggtgcgc
      781 tcctggatgt agccctcggg catggcgctc ttgaagaagt cgttcatctt catgtgctcg
      841 gggtagcggg
//
