LOCUS       sequence-123214-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for split-TEV-N fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-nTEV
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    103..327
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     316..333
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(790..807)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(790..806)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(799..821)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctgacgc gtccatagag cccaccgcat
      121 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
      181 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
      241 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
      301 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcggcggccg ctcaggtctg
      361 gaagttggtg gtcaccaggc agattctctc ctctctctgg ggctctctga acttcagctt
      421 ctgggggaag ggggggaagt ccttgggcat tctgatgatg atcatgtctc tgccgtcgat
      481 caggtgctgc tgcagggtgg tggtgttctt caccttgaac acgccgtgca ggctctgcac
      541 cagcagggtg ccgttgtttc ttctgaacag gtgcttgttg gtgatgatga aggggccgaa
      601 gccgatgccg tacaggctgg tggtgtggcc gtcgctctcg ttggtcaggt ggcagatggt
      661 gctgctgatg gggttgtagt ctctggggcc cttgaacagg ctctcgccgg atccccctcc
      721 actgccacca gaccccccgc tttttgggta ctggcccgct agctatcatt aggtaccgag
      781 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      841 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
//
