LOCUS       sequence-123218-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing EFS for generation of GMAP-compatible 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO EFS
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(376..678)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(478..497)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     801..823
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     815..832
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     816..832
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     839..858
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        839..857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actgcggcat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
       61 gcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
      121 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
      181 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
      241 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
      301 ccgtttctgc ggactggctt tctacgtgaa aaggatctag gtgaagatcc tttttgataa
      361 tctcatgcct gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
      421 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat
      481 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
      541 ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgccccgg
      601 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
      661 ggtgtaaacc ttaaactgcc gtacgtatag gctgcgcaac tgttgggaag ggcgatcggt
      721 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
      781 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
      841 atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg
      901 tgatggatat ctgcagaatt ccagcacact ggcggccgtt actagtggat cccgcttaag
      961 ctga
//
