LOCUS       sequence-123225-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for fusion tagging allowing inducible 
            destabilization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-ProteoTuner DD
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..414)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     361..380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(599..690)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
       61 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      121 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      181 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      241 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      301 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      361 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      421 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      481 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      541 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      601 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      661 tatttgaatg tatttagaaa aataaacaaa taggggttcc
//
