LOCUS       sequence-123226-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for fusion tagging allowing inducible 
            destabilization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-ProteoTuner DD
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    101..325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     314..331
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(350..673)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     primer_bind     complement(755..772)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(755..771)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(764..786)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctgacgcgt ccatagagcc caccgcatcc
      121 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
      181 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
      241 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
      301 gatggctggc aactagaagg cacagtcgag gctgatcagc ggcggccgct cattccagtt
      361 ctagaagctc cacatcgaag acgagagtgg catgtggtgg gatgatgcct gggtgcccag
      421 tggcaccata ggcataatct ggagatatag tcagtttggc tccctgaccc acactcatct
      481 gggcaacccc ttcttcccag cctcggatca cctcctgctt gcctagcata aacttaaagg
      541 gcttgtttct gtcccgggag gagtcaactt tctttccatc tccaagcatc ccggtgtagt
      601 gcaccacgca ggtctggccg cgcttgggga aggtgcgccc gtctcctggg gagatggttt
      661 ccacctgcac tccggatccc cctccactgc caccagaccc cccgcttttt gggtactggc
      721 ccgctagcta tcattaggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg
      781 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      841 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
//
