LOCUS       sequence-123232-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for streptavidin binding peptide fusion 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-SBP
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(192..209)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(192..208)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(201..223)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        418..522
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctctctggc cctgggggtg gtgctccagt ctggctctca gctgctccag ctcgccggcc
       61 aggccctcca ccacgtggcc gcctctccag ccggtggtct tctcgtccat ggatccccct
      121 ccactgccac cagacccccc gctttttggg tactggcccg ctagctatca ttaggtaccg
      181 agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      241 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      301 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
      361 tattttctcc ttacgcatct gtgcggtatt tcacaccgca cttttcgggg aaatgtgcgc
      421 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      481 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      541 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      601 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      661 ctggatctca acagcggtaa gatccttgag agttttcgcc
//
