LOCUS       sequence-123234-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for TagBFP fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagBFP
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..771
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(790..807)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggtacctaat gatagctagc gggccagtac ccaaaaagcg gggggtctgg tggcagtgga
       61 gggggatcca tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
      121 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
      181 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
      241 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
      301 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
      361 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
      421 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
      481 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
      541 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca
      601 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
      661 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
      721 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatta agcggccgcc
      781 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
      841 tgccttcctt gaccctggaa ggtgccactc ccactgtcct tt
//
