LOCUS       sequence-123235-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for TagBFP fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagBFP
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(186..290)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     485..507
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     499..516
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     500..516
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
       61 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
      121 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
      181 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
      241 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
      301 cgaaaagtgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
      361 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      421 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      481 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacctaatga
      541 tagctagcgg gccagtaccc aaaaagcggg gggtctggtg gcagtggagg gggatccatg
      601 agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac
      661 catcacttca agtgcacatc cgagggcgaa ggcaagccct
//
