LOCUS       sequence-123237-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint universal donor for strep tag II fusion tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-StrepTag II
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(103..120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(103..119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        329..433
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(652..671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcgaactgg gggtggctcc aggatccccc tccactgcca ccagaccccc cgctttttgg
       61 gtactggccc gctagctatc attaggtacc gagctcgaat tcactggccg tcgttttaca
      121 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      181 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      241 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      301 ttcacaccgc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      781 agtaagagaa ttatgcagtg
//
