LOCUS       sequence-123243-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint frame selector +0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-Frame +0
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(260..335)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(356..604)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(414..433)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(584..604)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(631..647)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(663..685)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gtttaccgta atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
       61 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
      121 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
      181 taattgatta ctattaataa ctagacaata atcaatgtca acgcgtatat ctggcccgta
      241 catcggaatt cgaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta
      301 gccttatttt aacttgctat ttctagctct aaaaccccgc tttttgggta ctggcggtgt
      361 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
      421 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
      481 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa
      541 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
      601 cctcggatcc ggccggcgcc tagagaagga gtgagggctg gataaaggga ggatcgaggc
      661 ggggtcgaac gaggaggttc aagggggaga gacggggcgg atggaggaag aggaggcgga
      721 ggcttagggt gtacaaaggg cttgacccag
//
