LOCUS       sequence-123248-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: SP-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    103..127
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(132..701)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 accggtaggg atcgaaccct ttgtctgctc gaagcattaa ccctcactaa agggaagcgg
       61 ccttagttat tcaggtacct gcggccgctc tagatgatat caaccacttt gtacaagaaa
      121 gctgggtttc aaaacagaga tgtgtcgaag atggacagtc ctgtgctgat atgcatggca
      181 tgcaagaggc actcgtcgtt caggaaggta tccagaatct cgttcaattc cggggtcagg
      241 ggtgagtcca ggttcagatc ctcggtcatg gactcaagtg tggttgtcag ctcatccaga
      301 tggccccttg ggggcggatg ggaaaggtcc atttggccac agattgcagc ctcttccttc
      361 tggggaatca cagtatcggc catctcccga agggctttga cagcctggct cgtctcttca
      421 tcgggatcct ccaacaggtg actggcctcg ggagtcactg cgggcgctgg atccagtggc
      481 tgagggactg gtgccggggt cagtgacccg actggctcat gtactggacc ggttggtgtt
      541 ggtgcgaggc tggcggggag tgggcggttg gcccatggac ttcctggagg atgaaatggc
      601 cggattcgtt ttggctggca cacctcgcgg ccctcaaaca cgtcactaat agcggagccg
      661 gcctcaggct tcggcaaaaa catcccttcc ctggaatccc ggctgccgct gccagagccc
      721 agcaaggctg agaaatccat atcggcgata gagctgaagt cctcgtcgcc agacagcagt
      781 ccattaggca ggcctg
//
