LOCUS       sequence-123251-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing SaCas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: AAV_NLS-SaCas9-NLS-VPR
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgattctgc ccttgccctt ggccagattc aggatgtgct tcttgaaggt ttcgtagctg
       61 atcttgctgt cgctgctgct caggtactgg aatggggtcc ggttgccctt cttgctgttt
      121 tcttcctgct tcacgagcac cttgttgttg aagctgttgt cgaaggacac gcttctgggg
      181 atgatgtggt ccacctcata gttgaagggg ttgttcagca gatcttccag agggatggct
      241 tccaggctgt acaggcactt gccttcctgc atgtcgtgca gcttgatctt ctcgatcagg
      301 tacttggcgt tctctttgcc ggtggtccgg atgatttcct cgatccgctc gttggtctgc
      361 cggttccgct tctgcatctc gttgatcatt ttctgggcgt ccttggagtt cttctcgcgg
      421 gccagctcga taatgatgtc gttgggcagg ccgtacttct tgatgatggc gttgatcact
      481 ttgatgctct ggatgaagct tctcttcacg acggggctca ggatgaagtc gtccaccagg
      541 gtggtgggga tctctttctg ctgggacagg tccaccttct tgggcaccag cttcagccgg
      601 ttgaagatag cgatctggtt gtcgttggtg tgccacagct cgtccaggat caggttgatg
      661 gccttcaggc tcaggttgtg ggtgccggta tagcccttca gattagagat ctgctcgatc
      721 tcttcctggg tcagctcgga gttc
//
