LOCUS       sequence-123264-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing pGK for generation of GMAP-compatible 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO pGK
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(279..581)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(381..400)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     704..726
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     718..735
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     719..735
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     742..761
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        742..760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(927..946)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
       61 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
      121 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
      181 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gaaaaggatc
      241 taggtgaaga tcctttttga taatctcatg cctgacattt atattcccca gaacatcagg
      301 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
      361 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
      421 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg
      481 gggatcacca tccgtcgccc cggcgtgtca ataatatcac tctgtacatc cacaaacaga
      541 cgataacggc tctctctttt ataggtgtaa accttaaact gccgtacgta taggctgcgc
      601 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      661 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      721 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg ccctctagat
      781 gcatgctcga gcggccgcca gtgtgatgga tatctgcaga attccagcac actggcggcc
      841 gttactagtg gatcccgctt aagctgtagc gctaatgtcg ggtctcccgg agatgaggaa
      901 gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag
      961 gacctcgg
//
