LOCUS       sequence-123274-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses red fluorescent protein, mKate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_CAG:mKate
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(54..78)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          103..306
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(126..146)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tgtgcatgtt ctccttaatc agctcttcgc ccttagacac catggtggcg atcagcctgc
       61 ttttttgtac aaacttgggt cgaattcgcc cttgggatcc acctgaaatg gaagaaaaaa
      121 actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa
      181 ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt
      241 gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa
      301 gctcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc
      361 gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc
      421 cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc
      481 cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg
      541 cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct
      601 cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc
      661 gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac
      721 aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag
      781 agacttatcg aaagcagcga gacaggcgcg aa
//
