LOCUS       sequence-123279-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO backbone for GMAP genes with sites #2 and #5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO 2-5
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(379..681)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(481..500)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     804..826
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     818..835
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     819..835
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     842..861
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        842..860
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
       61 agagcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
      121 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
      181 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
      241 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
      301 gcaccgtttc tgcggactgg ctttctacgt gaaaaggatc taggtgaaga tcctttttga
      361 taatctcatg cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
      421 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
      481 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
      541 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
      601 cggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
      661 ataggtgtaa accttaaact gccgtacgta taggctgcgc aactgttggg aagggcgatc
      721 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
      781 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
      841 gtaatacgac tcactatagg gcgaattggg ccctctagat gcatgctcga gcggccgcca
      901 gtgtgatgga tatctgcaga attccagcac actggcggcc gttactagtg gatccagagt
      961 aattcaaccc caaacaacaa cgtttaaacc agctcgatgc ggttcaccag ggtgtcgccc
     1021 tcgaacttca cc
//
