LOCUS       sequence-123283-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of 
            GMAP-compatible gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO tTR-KRAB-rtTA3-Luc
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..666
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(119..138)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             667..687
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             790..984
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
ORIGIN
        1 cgagctcgga tcctaactcg aacgctagct gtgcgatcgt ttaccatggc tagattagat
       61 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
      121 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
      181 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
      241 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
      301 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
      361 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
      421 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
      481 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
      541 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
      601 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt
      661 gggtcgccaa aaaagaagag aaaggtcgac ggcggtggtg ctttgtctcc tcagcactct
      721 gctgtcactc aaggaagtat catcaagaac aaggagggca tggatgctaa gtcactaact
      781 gcctggtccc ggacactggt gaccttcaag gatgtatttg tggacttcac cagggaggag
      841 tggaagctgc tggacactgc tcaacaaatc gtgtacagaa atgtgatgct ggagaactat
      901 aagaacctgg tttccttggg ttatcagctt actaagccag atgtgatcct ccggttggag
      961 aagggagaag agccctggct ggtggagaga gaaatt
//
