LOCUS       sequence-123284-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of 
            GMAP-compatible gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO tTR-KRAB-rtTA3-Luc
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..99)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(115..819)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atgggcccgg ggttttcttc
       61 cacgtctcct gcttgcttta acagagagaa gttcgtggct ccggatccta acttcccggg
      121 gagcatgtca aggtcaaaat cgtcaagggc atcggctggg agcatgtcta agtcaaaatc
      181 gtcaagggcg tcggtcggcc cgccgctttc gcactttagc tgtttctcca ggccacatat
      241 gattagttcc aggccgaaaa ggaaggcagg ttcggctccc tgccggtcga acagctcaat
      301 tgcttgtctc agaagtgggg gcatagaatc ggtggtaggt gtctctcttt cctcttttgc
      361 tacttgatgc tcctgttcct ccaatacgca gcccagtgta aagtggccca cggcggacag
      421 agcgtacagt gcgttctcca gggagaagcc ttgctgacac aggaacgcga gctgattttc
      481 cagggtttcg tactgtttct ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg
      541 atgtgagagg agagcacagc ggtatgactt ggcgttgttc cgcagaaagt cttgccatga
      601 ctcgccttcc agggggcaga agtgggtatg atgcctgtcc agcatctcga ttggcagggc
      661 atcgagcagg gcccgcttgt tcttcacgtg ccagtacagg gtaggctgct caactcccag
      721 cttttgagcg agtttccttg tcgtcaggcc ttcgataccg acaccattga gtaattccag
      781 agctccgttt atgactttgc tcttgtccag tctagacatt ggacccgggt tctcttcgac
      841 g
//
