LOCUS       sequence-123285-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Redox-Sensitive Red Fluorescent Protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Fan Y, Chen Z, Ai HW
  TITLE     Monitoring redox dynamics in living cells with a redox-sensitive red
            fluorescent protein.
  JOURNAL   Anal Chem. 2015 Mar 3;87(5):2802-10. doi: 10.1021/ac5041988. Epub 
            2015 Feb 20.
  PUBMED    25666702
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac5041988";
            journalName: "Anal Chem"; date: "2015-03-3- 3"; volume: "87"; issue:
            "5"; pages: "2802-10"
COMMENT     Sequence Label: pcDNA3-rxRFP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             110..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(146..154,155..169)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc gccaccatgg gaggttctca tcatcatcat catcatggta tggctagcat
      121 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgagctc
      181 gagcgagctt agttgcgcta taggtcggct gagctcaccc gtggtttccg agcggatgta
      241 ccccgaggac ggcgccctga agagcgagat caagaagggg ctgaggctga aggacggcgg
      301 ccactacgcc gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      361 cgcctacatc gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt
      421 ggaacagtgc gaacgcgccg agggccgcca ctccaccggc gacatggacg agctgtacaa
      481 gggaggtaca ggcgggagtc tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
      541 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      601 cgagggcgag ggcgagggcc gcccctacga ggcctttcag accgctaagc tgaaggtgac
      661 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      721 caaggcctac attaagcacc cagccgacat ccccgactac ttcaagctgt ccttccccga
      781 gggcttcagg tgggagcgcg tgatgaactt cgaggacggc ggcattattc acgttaacca
      841 ggactcctcc ctgcaggacg gcgtattcat ctacaaggtg aagctgcgcg gcaccaactt
//
