LOCUS       sequence-123297-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  redox-sensitive red fluorescent protein rxRFP1.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Fan Y, Ai HW
  TITLE     Development of redox-sensitive red fluorescent proteins for imaging 
            redox dynamics in cellular compartments.
  JOURNAL   Anal Bioanal Chem. 2016 Jan 12.
  PUBMED    26758595
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Bioanal Chem. 2016 Jan 12."
COMMENT     Sequence Label: pBAD-rxRFP1.2
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..99
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             103..135
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(139..147,148..162)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tgacgctttt ttcgcaactc tctactgttt ctccataccc gttttttggg ctaacaggag
       61 gaattaacca tggggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      121 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatccgag ctcgagcgag
      181 cttagttgcg ctataggtcg gctgagctca cccgtggttt ccgagcggat gtaccccgag
      241 gacggcgccc tgaagagcga gatcaagaag gggctgaggc tgaaggacgg cggccactac
      301 gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      361 atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg actacaccat cgtggaacag
      421 tgcgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggaggt
      481 acaggcggga gtctggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      541 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      601 gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt gaccaagggt
      661 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      721 tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc cgagggcttc
      781 aggtgggagc gcgtgatgaa
//
