LOCUS       sequence-123304-         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 
            driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     180..198
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    complement(230..263)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    379..448
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 aaggaggcaa acagcgctga ctttgagtgg aatgtcattt tgagtgagag gtaatcgaaa
       61 gaacctggta catcaaatac ccttggatcg aagtaaattt aaaactgatc agataagttc
      121 aatgatatcc agtgcagtaa aaaaaaaaaa tgtttttttt atctactttc cgcaaaaatg
      181 ggttttatta acttacatac atactagaat taggccttct agtggatcca taacttcgta
      241 tagcatacat tatacgaagt tatggcgcaa aggtttttaa ttgcatttga tttgtttctg
      301 gcagggggaa agtcgaaaaa tctgctaaca atttgcatta ctccattggg tattaaaatt
      361 gaagatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc
      421 cccgggcgcg tactccacct cacccatctg gtccatcatg atggtcgaca agcttagaag
      481 ttcaaatggg ctttcatatt tacttttttc tctgagtggg taaggtcatt gtggggtctt
      541 aagggctaaa gggtagtgat gatgaaggca tttgatgcca ttgttcaata acttacctgc
      601 cggaacctcc gcttccaccg ctacct
//
